Solution Information | help | |
Enzyme: | Serine/threonine-protein kinase B-raf [V600E] | |
inhibitor: | BDBM29706 | |
substrate: | Dual specificity mitogen-activated protein kinase kinase 1 | |
Solution Type: | Aqueous | |
pH at Preparation: | 7.2 | |
Temp. Prep.: | 22 | |
Comments: | n/a | |
Solute 1: | MgCl2 | |
Concentration: | 10 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 2: | ATP | |
Concentration: | 100 uM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 3: | beta-glycerophosphate | |
Concentration: | 25 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 4: | beta-ME | |
Concentration: | 0.1 % | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 1: | water | |
Concentration: | 1.0 | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
pH Buffer 1: | MOPS | |
Concentration: | 20 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
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